Friday, April 1, 2011

Bartonella Henselae

While doing a virtual lab on bacterial DNA, I was able to be a lab technician in a molecular biology lab and was responsible for providing lab results for use in diagnosing patients. On the certain DNa that I found, it came up with the bacteria Bartonella Henselae. Here is what was found:
Bartonella henselae Various species of Bartonella that are pathogenic to humans are transmitted via a vector, or directly from an animal reservoir. For example, B. bacilliformis via sandflies causes Oroya fever; B. quintana via body lice causes trench fever; and B. henselae via cats causes cat scratch disease (CSD). CSD typically manifests as swellings of the lymph glands, possibly with skin lesions at the site of inoculation and possibly accompanied by fever, fatigue, and other symptoms. Immunocompromised patients may be particularly susceptible and can develop a different disease, bacillary angiomatosis, as a result of infection by B. henselae or B. quintana.

Below are pictures of Bartonella Henselae:

How I was able to sequence the DNA:

In the first step, my objective was to:
  • Prepare a sample from a patient and isolate whole bacterial DNA.
  • Make many copies of the desired piece of DNA.
  • Sequence the DNA.
  • Analyze the sequence and identify the bacteria.

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